Mycobacterium avium subspecies paratuberculosis (MAP) is a Gram positive, acid-fast bacterium which causes Johne’s disease or Paratuberculosis in domestic livestock species. For screening of cows for MAP infection a total of 103 milk samples from individual cows were collected from organized (57) and unorganized (46) farms. Presence of anti-MAP antibodies in milk were screened by indirect enzyme linked immunosorbent assays (iELISA) test, 72 () cow milk samples were found positives ( from organized farms and from unorganized farms). Positivity percentages were in Gir cows and in non-descriptive and crossbred cows irrespective of the farming system for the presence of MAP antibodies. | Serological microscopical and molecular examinations of cow milk samples for diagnosis of Paratuberculosis at mhow of Madhya Pradesh in India
