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Mechanical transmission of leaf crinkle disease in urdbean (Vigna mungo (L.) Hepper) by sprout seed abrasion method

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In the present study, seeds were mechanically sap inoculated by sprout seed abrasion method. Influence of artificial inoculation on ULCD incidence has been studied in CO5 cultivar of blackgram. Symptoms started appearing 30-35 DPI and disease incidence was up to43.3%. This protocol will help in maintenance of pure inoculum of ULCD under glasshouse conditions and further investigations are under process to standardize efficient protocol to obtain higher transmission efficiency. |

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Mechanical transmission of leaf crinkle disease in urdbean (Vigna mungo (L.) Hepper) by sprout seed abrasion method
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