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Negative regulation of diminutive cancer regulator through differentiation and microRNA pathway components in Drosophila cells

Drosophila model is intensively studied for the development of cancer. The diminutive (dMyc), a homolog of the human MYC gene, is responsible for cell- apoptosis and its upregulation is responsible for determining the fate of cancerous growth in humans and Drosophila model. This work implores the requirement of dMyc and its expression as one of the major regulator of cancer with other proteins and repression of dMyc mRNA in Drosophila S2 cells. Here we report protein complex of Argonaute 1 (AGO1), Bag of marbles (Bam), and Brain tumor (Brat) proteins and not the individual factor of this complex repression of dMyc mRNA in Drosophila Schneider 2 cells and promote differentiation in cystoblast of Drosophila ovary. | Turkish Journal of Biology Turk J Biol 2021 45 180-186 http journals.tubitak.gov.tr biology TÜBİTAK Research Article doi 10.3906 biy-2012-4 Negative regulation of diminutive cancer regulator through differentiation and microRNA pathway components in Drosophila cells 1 2 Sumira MALIK 1 Amity institute of Biotechnology Amity University Jharkhand Ranchi Jharkhand India 2 School of Biological Sciences and Technology Chonnam National University the Republic of Korea Received 02.12.2020 Accepted Published Online 26.02.2021 Final Version 20.04.2021 Abstract Drosophila model is intensively studied for the development of cancer. The diminutive dMyc a homolog of the human MYC gene is responsible for cell- apoptosis and its upregulation is responsible for determining the fate of cancerous growth in humans and Drosophila model. This work implores the requirement of dMyc and its expression as one of the major regulator of cancer with other proteins and repression of dMyc mRNA in Drosophila S2 cells. Here we report protein complex of Argonaute 1 AGO1 Bag of marbles Bam and Brain tumor Brat proteins and not the individual factor of this complex repression of dMyc mRNA in Drosophila Schneider 2 cells and promote differentiation in cystoblast of Drosophila ovary. These results exhibit the significant role of this complex including master differentiation factor Bam with other various differentiation factor Brat and microRNA pathway component AGO1 which may negatively regulate dMyc mRNA and so the dMyc protein. Key words Bam Brat AGO1 microRNA dMyc 1. Introduction in cystoblast individually Harris et al. 2011 Daneshwar In the Drosophila ovary the balance between symmetric et al. 2013 . Bam was the first intrinsic responsible for CB and asymmetric divisions is tightly regulated to control differentiation McKearin and Spradling 1990 Ohlstein and produce appropriate amounts of proliferating stem et al. 2000 . Bam and benign gonial cell neoplasm bgcn cells and differentiated cells. The