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Identification and in silico analysis of PHANTASTICA gene in Saintpaulia ionantha H. Wendl (Gesneriaceae)

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Saintpaulia ionantha H. Wendl (synonym Streptocarpus ionanthus) has been considered an ornamental plant. The PHANTASTICA (PHAN) gene has a significant role in the formation of plant organs adaxial–abaxial polarity. In the present study the PHAN gene was identified in Saintpaulia for the first time using PCR. To determine the expression pattern of the PHAN gene, gene expression was compared at 3 developmental stages using real time PCR. For in silico analyses, protein characteristics, secondary and 3D structures of protein, intracellular localization, and the phylogenetic tree were investigated using bioinformatics tools. The partial CDS of PHAN gene contained 756 nucleotides, a predicted protein encoded with 252 amino acids with a molecular weight of 28,474 Da. |

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