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Associations of otubain 1, stat, shp and NF-KB2 expression with clinical features in NON-HODGKIN’s lymphoma patients

In this study, all the expressions of Otubain-1, NF-κB2, SHPs and STATs genes, the concentrations of cytokines IL-1β, IL-6 and TNF-α and clinical features in NHL patients were examined. To the end, gene expression levels of 82 NHL patients and 56 healthy individuals were determined by quantitative real time RT-PCR and secretion of cytokines by ELISA. | ACADEMIA JOURNAL OF BIOLOGY 2024 46 1 69 78 DOI 10.15625 2615-9023 19064 ASSOCIATIONS OF OTUBAIN-1 STAT SHP AND NF-KB2 EXPRESSION WITH CLINICAL FEATURES IN NON-HODGKIN s LYMPHOMA PATIENTS Do Thi Trang1 Nguyen Trong Ha2 Nguyen Thi Xuan1 3 1 Institute of Genome Research Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Ha Noi Vietnam 2 103 Hospital Vietnam Military Medical University 261 Phung Hung Ha Dong Ha Noi Vietnam 3 Graduate University of Science and Technology Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Ha Noi Vietnam Received 3 October 2023 accepted 6 March 2024 ABSTRACT Non-Hodgkin s lymphoma NHL is a group of lymphoproliferative disorders characterized by the abnormal proliferation and accumulation of lymphocytes in the lymphatic system. An ovarian tumor domain containing ubiquitin aldehyde binding protein 1 Otubain-1 is a deubiquitinating enzyme that cleaves ubiquitin or ubiquitin-like molecules and is expressed in various human tissues. The pathogenesis of NHL is associated with activations of the nuclear transcription factor NF-κB and signal transducer and activator of transcription proteins JAK STAT signaling pathways. In this study all the expressions of Otubain-1 NF-κB2 SHPs and STATs genes the concentrations of cytokines IL-1β IL-6 and TNF-α and clinical features in NHL patients were examined. To the end gene expression levels of 82 NHL patients and 56 healthy individuals were determined by quantitative real time RT-PCR and secretion of cytokines by ELISA. As a result concentrations of IL-6 and TNF-α in the patient group were found higher than in the healthy individuals and patients with higher LDH concentrations in the clinical cutoff 280 U L showed increased concentrations of AST ALT and GGT than those with normal LDH concentrations. Interestingly NHL patients with high Otubain-1 expression had significant elevations of GGT and ferritin concentrations as well as STAT-5 expression compared to those with low Otubain-1 .