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Báo cáo khoa học: "Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis | J. Vet. Sci. 2000 1 2 77-80 OURNAL OF Veterinary Science Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis I rjSkliin 1 3 Tlíinííd A AÌiìC ÌTằế t1 2 IX Tí Ti C diiti n1 2 I-Ií lí nA Addiìíl1 3 IX Tovb VAZdlt AV1 3 Taekyun Shin aniel Weinstock. Aailehe asno Helene Acland ivAaik.wahei Hyun Young Kim1 3 and H.Graham Purchase1 3 Department of Veterinary Medicine Institute for Life Science BK 21 Cheju National University Cheju 690-756 Korea Pennsylvania Animal Diagnostic Laboratory System Center for Veterinary Diagnostics and Investigation Pennsylvania State University University Park PA. U.S.A. Pennsylvania Veterinary Laboratory Harrisburg PA. U.S.A. The expression of both constitutive and inducible forms of nitric oxide synthase NOS was investigated by immunohistochemical staining of formalin-fixed paraffin-embedded sections in normal and Listeria monocytogenes-infected brains of goats. In normal control goats a small number of neurons showed immunoreactivity of both iNOS and nNOS and the number of iNOS-positive neurons was higher than the number of nNOS-positive neurons. In natural listeriosis listeria antigens were easily immunostained in the inflammatory cells of microabscesses. In this lesion the immunoreactivity of iNOS in neurons was more intense than the control but nNOS was not. In microabscesses nNOS was weakly visualized in macrophages and neutrophils while iNOS was expressed in macrophages but not in neutrophils. These findings suggest that normal caprine brain cells including neurons constitutively express iNOS and nNOS and the expressions of these molecules is increased in Listeria monocytogenes infections. Furthermore inflammatory cells including macrophages expressing both nNOS and iNOS may play important roles in the pathogenesis of bacterial meningoencephalitis in goat. Key words nitric oxide synthase goat brain listeriosis Introduction Nitric oxide NO