Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo khoa học: "Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development | J. Vet. Sci. 2005 6 2 97-101 JOURNAL OF Veterinary Science Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development Heechul Kim Changjong Moon Meejung Ahn Yongduk Lee1 Hwanglyong Kim Seungjoon Kim 7 CM o 7 Ơ o 7 CT 7 CT v CT 7 CTt 7 Taeyoung Ha2 Youngheun Jee Taekyun Shin Department of Veterinary Medicine Graduate School Cheju National University Jeju 690-756 Korea 2Korea Racing Association Gwacheon 427-711 Korea The expression of nitric oxide synthase NOS isoforms in the ovaries of pigs was examined to study the involvement of nitric oxide a product of NOS activity in the function of the ovary. Western blot analysis detected three types of NOS in the ovary including constitutive neuronal NOS nNOS endothelial NOS eNOS and inducible NOS iNOS eNOS immunoreactivity was more intense compared with that of iNOS or nNOS. Immunohistochemical studies demonstrated the presence of nNOS and eNOS in the surface epithelium stroma oocytes thecal cells and endothelial cells of blood vessels. Positive immunoreactions for nNOS and iNOS were detected in the granulosa cells from multilaminar and antral follicles but not in those of unilaminar follicles. iNOS was detected in the surface epithelium oocytes and theca of multilaminar and antral follicles. Taking all of the findings into consideration the observed differential expression of the three NOS isoforms in the ovary suggests a role for nitric oxide in modulating reproduction in pigs. Key words granulosa cell nitric oxide synthase oocyte ovary pig Introduction Nitric oxide NO is a reactive free radical gas that is derived from L-arginine by the action of NO synthase NOS 15 . NO has diverse roles including intracellular signaling and vasoregulation 1 and exists in a variety of isoforms. A constitutive calcium-dependent isoform cNOS is activated rapidly by agonists that elevate intracellular free calcium and is found in endothelial cells eNOS and the brain nNOS 7 . A calcium-independent .