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Báo cáo khoa học: "Development of synthetic peptide ELISA based on nonstructural protein 2C of foot and mouth disease virus"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Development of synthetic peptide ELISA based on nonstructural protein 2C of foot and mouth disease virus | J. Vet. Set. 2005 6 4 317-325 JOURNAL OF Veterinary Science Development of synthetic peptide ELISA based on nonstructural protein 2C of foot and mouth disease virus Jae Ku Oem Soo Jeong Kye1 Kwang Nyeong Lee1 Jong Hyeon Park1 Yong Joo Kim1 Hee Jong Song2 Max Yeh3 1 National Veterinary Research and Quarantine Service Ministry of Agriculture Anyang 430-824 Korea department of Veterinary infectious Disease College of Veterinary Medicine Chonbuk National University Jeonju 561-756 Korea 3Plum Island Animal Disease Center Animal Plant Health Inspection Service USDA Greenport NY 11944 USA It was reported that the sera of convalescent animals contain antibodies to foot and mouth disease FMD virus FMDV 2C highly conserved nonstructural protein NSP whereas the sera of vaccinated animals do not. But ELISA methods using this protein were not reported and developed until recently. In this study NSP 2C peptides were synthesized within the amino acid sequence of the conserved 2C nonstructural region of FMDV according to the sequences from Genbank database and used for identifying antigenic determinants. One of the synthesized thirteen peptides gave strong positive reactivity with most of the sera from 13 FMD infected farms but not with sera from vaccinated and non-infected animals. Moreover with the sera collected through serial bleedings from four cattle and five goats infected with FMDV 0 SKR 2000 experimentally positive results were obtained in two species after 10 days post infection DPI . Therefore we tried to develop and evaluate this ELISA based on 2C peptides. In comparison with the commercial NSP ELISA the 2C peptide based ELISA method showed good specificity and sensitivity. These results demonstrate that the synthetic 2C peptide ELISA can be a complementary marker to differentiate FMDV-infected from vaccinated on a herd basis. Key words foot and mouth disease virus FMDV nonstruc-tural protein 2C peptide ELISA Introduction Foot and mouth disease FMD is a highly .