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Báo cáo y học: "Controlling Osteogenesis and Adipogenesis of Mesenchymal Stromal Cells by Regulating A Circadian Clock Protein with Laser Irradiation"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Controlling Osteogenesis and Adipogenesis of Mesenchymal Stromal Cells by Regulating A Circadian Clock Protein with Laser Irradiation. | Int. J. Med. Sci. 2008 5 319 International Journal of Medical Sciences ISSN 1449-1907 www.medsci.org 2008 5 6 319-326 Ivyspring International Publisher. All rights reserved Research Paper Controlling Osteogenesis and Adipogenesis of Mesenchymal Stromal Cells by Regulating A Circadian Clock Protein with Laser Irradiation Toshihiro Kushibiki1 2 H and Kunio Awazu3 1. Frontier Research Center Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita Osaka 565-0871 Japan 2. PRESTO Japan Science and Technology Agency 4-1-8 Honcho Kawaguchi Saitama 332-0012 Japan 3. Division of Sustainable Energy and Environmental Engineering Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita Osaka 565-0871 Japan H Correspondence to Toshihiro Kushibiki kushibiki@see.eng.osaka-u.ac.jp Phone Fax 81-6-6878-6330 Received 2008.09.16 Accepted 2008.10.23 Published 2008.10.26 Mesenchymal stromal cells MSCs are multipotent cells present in adult bone marrow that replicate as undifferentiated cells and can differentiate to lineages of mesenchymal tissues. Homeostatic control of bone remodelling maintains bone mass by insuring that bone resorption and bone formation occur sequentially and in a balanced manner. As most homeostatic functions occur in a circadian manner a circadian clock could control bone mass. Here we show that laser irradiation can direct the osteogenesis and adipogenesis of mouse MSCs by altering the intracellular localization of the circadian rhythm protein Cryptochrome 1 mCRY1 . After laser irradiation wavelength 405 nm to MSCs circadian rhythm protein mCRY1 and mPER2 were immunostained and histochemical stainings for osteogenic or adipogenic differentiation were observed. Laser irradiation promoted osteogenesis and reduced adipogenesis of MSCs induced the translocation of mCRY1 and mPER2 protein from the cytoplasm to the nucleus and decreased mCRY1 mRNA levels quantified by real-time PCR. Since the timing of nuclear accumulation of clock proteins .