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Báo cáo y học: "High CXCR3 expression in synovial mast cells associated with CXCL9 and CXCL10 expression in inflammatory synovial tissues of patients with rheumatoid arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: High CXCR3 expression in synovial mast cells associated with CXCL9 and CXCL10 expression in inflammatory synovial tissues of patients with rheumatoid arthritis. | Available online http arthritis-research.eom content 5 5 R241 Research article Open Access High CXCR3 expression in synovial mast cells associated with CXCL9 and CXCL10 expression in inflammatory synovial tissues of patients with rheumatoid arthritis Peter Ruschpler1 Peter Lorenz2 Wolfram Eichler3 Dirk Koczan2 Claudia Hanel1 Roger Scholz4 Christian Melzer5 Hans-Jurgen Thiesen2 and Peter Stiehl1 Institute of Pathology University of Leipzig Leipzig Germany institute of Immunology University of Rostock Rostock Germany 3Eye Hospital University of Leipzig Leipzig Germany 4Department of Orthopedic Surgery University of Leipzig Leipzig Germany 5Specialty Hospital of Orthopedic and Trauma Surgery Waldkrankenhaus Bad Duben Germany Correspondence Peter Ruschpler e-mail rusp@medizin.uni-leipzig.de Received 11 Nov 2002 Revisions requested 8 Jan 2003 Revisions received 6 May 2003 Accepted 14 May 2003 Published 26 Jun 2003 Arthritis Res Ther 2003 5 R241-R252 DOI 10.1186 ar783 2003 Ruschpler et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract To improve our knowledge on the pathophysiology of rheumatoid arthritis RA we investigated gene expression patterns in synovial tissue from RA and osteoarthritis OA patients. DNA oligonucleotide microarray analysis was employed to identify differentially expressed genes in synovial tissue from pathologically classified tissue samples from RA n 20 and OA patients n 10 . From 7131 gene sets displayed on the microarray chip 101 genes were found to be upregulated and 300 genes to be downregulated in RA as compared with OA. Semiquantitative reverse-transcription polymerase chain reaction Western blotting and immunohistochemistry were used to validate microarray expression levels. These experiments revealed that .