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Báo cáo y học: " Intramuscular electroporation with the pro-opiomelanocortin gene in rat adjuvant arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Intramuscular electroporation with the pro-opiomelanocortin gene in rat adjuvant arthritis. | Available online http arthritis-research.eom content 6 1 R7 Research article Open Access Intramuscular electroporation with the pro-opiomelanocortin gene in rat adjuvant arthritis I-Chuan Chuang1 Chien-Ming Jhao2 Chih-Hsun Yang3 Hsien-Chang Chang1 Chien-Wen Wang1 Cheng-Yuan Lu3 Yao-Jen Chang3 Sheng-Han Lin3 Pao-Lin Huang4 and Lin-Cheng Yang4 institute of Biomedical Engineering National Cheng Kung University Tainan Taiwan institute of Biomedical Engineering National Taiwan University Hospital Taipei Taiwan 3Gene Therapy Laboratory Chang Gung Memorial Hospital Kaohsiung 833 Taiwan 4Gene Therapy Laboratory Tajen Institute of Technology Pingtung 907 Taiwan Corresponding author Lin-Cheng Yang e-mail lcyang1@ms13.hinet.net Received 14 Aug 2003 Revisions requested 17 Sep 2003 Revisions received 18 Sep 2003 Accepted 30 Sep 2003 Published 17 Oct 2003 Arthritis Res Ther 2004 6 R7-R14 DOI 10.1186 ar1014 2004 Chuang et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Endogenous opioid peptides have an essential role in the intrinsic modulation and control of inflammatory pain which could be therapeutically useful. In this study we established a muscular electroporation method for the gene transfer of proopiomelanocortin POMC in vivo and investigated its effect on inflammatory pain in a rat model of rheumatoid arthritis. The gene encoding human POMC was inserted into a modified pCMV plasmid and 0-200 ig of the plasmid-POMC DNA construct was transferred into the tibialis anterior muscle of rats treated with complete Freund s adjuvant CFA with or without POMC gene transfer by the electroporation method. The safety and efficiency of the gene transfer was assessed with the following parameters thermal hyperalgesia serum adrenocorticotropic hormone .