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Báo cáo y học: "Circulating tumour necrosis factor-α bioactivity in rheumatoid arthritis patients treated with infliximab: link to clinical respone"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Circulating tumour necrosis factor-α bioactivity in rheumatoid arthritis patients treated with infliximab: link to clinical response. | Available online http arthritis-research.eom content 7 1 R149 Research article Circulating tumour necrosis factor-a bioactivity in rheumatoid arthritis patients treated with infliximab link to clinical response Hubert Marotte1 Wlodzimierz Maslinski2 and Pierre Miossec1 Open Access Departments of Immunology and Rheumatology and Unite Mixte Hospices Civils de Lyon-BioMérieux Hôpital Edouard Herriot Lyon France institute of Rheumatology University of Warsaw Warsaw Poland Corresponding author Pierre Miossec miossec@univ-lyon1.fr Received 14 Apr 2004 Revisions requested 7 May 2004 Revisions received 18 Sep 2004 Accepted 25 Oct 2004 Published 1 Dec 2004 Arthritis Res Ther 2005 7 R149-R155 DOI 10.1186 ar1465 2004 Marotte et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is cited. Abstract Our objective was to clarify the heterogeneity in response to infliximab treatment in rheumatoid arthritis RA to this end a bioassay was designed to explore the contribution of circulating tumour necrosis factor TNF -a bioactivity and its possible link to response. The bioassay is based on the induction of IL-6 and osteoprotegerin OPG production by synoviocytes in response to TNF-a. RA synoviocytes were cultured with TNF-a 5 ng ml and 42 RA plasma samples collected just before starting therapy. Levels of IL-6 and OPG were measured in supernatants. In 20 of the patients plasma samples collected before and 4 hours after the first and the ninth infusions were tested in the same way. Plasma concentrations of TNF-a and p55 and p75 soluble receptors were measured using ELISA. TNF-a induced IL-6 and OPG production by synoviocytes which was further increased with patient plasma dilutions and inhibited by infliximab. With plasma samples obtained before the first .