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Báo cáo y học: "Potential involvement of oxidative stress in cartilage senescence and development of osteoarthritis: oxidative stress induces chondrocyte telomere instability and downregulation of chondrocyte function"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Potential involvement of oxidative stress in cartilage senescence and development of osteoarthritis: oxidative stress induces chondrocyte telomere instability and downregulation of chondrocyte function. | Available online http arthritis-research.eom content 7 2 R380 Research article Potential involvement of oxidative stress in cartilage senescence and development of osteoarthritis oxidative stress induces chondrocyte telomere instability and downregulation of chondrocyte function Kazuo Yudoh Nguyen van Trieu Hiroshi Nakamura Kayo Hongo-Masuko Tomohiro Kato and Kusuki Nishioka Open Access Department of Bioregulation Institute of Medical Science St. Marianna University Kawasaki City Japan Corresponding author Kazuo Yudoh yudo@marianna-u.ac.jp Received 13 Nov 2003 Revisions requested 4 Dec 2003 Revisions received 25 Nov 2004 Accepted 10 Dec 2004 Published 26 Jan 2005 Arthritis Res Ther 2005 7 R380-R391 DOI 10.1186 ar1499 2005 Yudoh et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Oxidative stress leads to increased risk for osteoarthritis OA but the precise mechanism remains unclear. We undertook this study to clarify the impact of oxidative stress on the progression of OA from the viewpoint of oxygen free radical induced genomic instability including telomere instability and resulting replicative senescence and dysfunction in human chondrocytes. Human chondrocytes and articular cartilage explants were isolated from knee joints of patients undergoing arthroplastic knee surgery for OA. Oxidative damage and antioxidative capacity in OA cartilage were investigated in donor-matched pairs of intact and degenerated regions of tissue isolated from the same cartilage explants. The results were histologically confirmed by immunohistochemistry for nitrotyrosine which is considered to be a maker of oxidative damage. Under treatment with reactive oxygen species ROS 0.1 pmol l H2O2 or an antioxidative agent ascorbic acid .