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Báo cáo y học: "The mechanism of low-concentration sodium nitroprusside-mediated protection of chondrocyte deat"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:The mechanism of low-concentration sodium nitroprusside-mediated protection of chondrocyte death. | Available online http arthritis-research.eom content 7 3 R526 Research article The mechanism of low-concentration sodium nitroprusside-mediated protection of chondrocyte death Hyun A Kim1 Ki Byoung Lee2 and Sang-cheol Bae3 Department of Internal Medicine Hallym University Sacred Heart Hospital Kyunggi-do Korea 2Department of Orthopedic Surgery Hallym University Sacred Heart Hospital Kyunggi-do Korea 3Department of Internal Medicine Hanyang University College of Medicine Seoul Korea Corresponding author Hyun A Kim kimha@hallym.ac.kr Received 26 Nov 2004 Revisions requested 22 Dec 2004 Revisions received 22 Jan 2005 Accepted 1 Feb 2005 Published 1 Mar 2005 Arthritis Research Therapy 2005 7 R526-R535 DOI 10.11 86 ar1 705 This article is online at http arthritis-research.com content 7 3 R526 2005 Kim et al. licensee BioMed Central Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Sodium nitroprusside SNP a widely used nitric oxide donor has recently been shown to mediate chondrocyte apoptosis by generating reactive oxygen species whereas more potent nitric oxide donors do not induce chondrocyte apoptosis. The present study was performed to investigate the protective effect of a low concentration of SNP upon the cytotoxicity of chondrocytes to higher concentrations of SNP and to elucidate the underlying mechanism. Human osteoarthritis chondrocytes were cultured as monolayers and first-passage cells were used for the experiments. Chondrocyte death induced by 1 mM SNP was completely inhibited by pretreating with 0.1 mM SNP. This protective effect of SNP was replicated by the guanosine-3 5 K-cyclic monophosphate analog DBcGMP. Protection from chondrocyte death conferred by 0.1 mM SNP was mediated by heme oxygenase 1 HO-1 as was revealed