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Báo cáo y học: "Application of in vivo microscopy: evaluating the immune response in living animals"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Application of in vivo microscopy: evaluating the immune response in living animals. | Arthritis Research Therapy December 2005 Vol 7 No 6 Scheinecker Review Application of in vivo microscopy evaluating the immune response in living animals Clemens Scheinecker Department of Rheumatology Internal Medicine III Medical University of Vienna MUW General Hospital of Vienna AKH Waehringer Guertel 18-20 A-1090 Wien Austria Corresponding author Clemens Scheinecker clemens.scheinecker@meduniwien.ac.at Published 19 October 2005 This article is online at http arthritis-research.com content 7Z6 246 2005 BioMed Central Ltd Arthritis Research Therapy 2005 7 246-252 DOI 10.1186 ar1843 Abstract The initiation of an immune response requires that professional antigen-presenting cells such as dendritic cells physically interact with antigen-specific T cells within the complex environment of the lymph node. Although the way in which antigen is presented to T cells and in particular the cellular associations involved in antigenspecific stimulation events have been extensively investigated data on antigen presentation have come primarily from studies in vitro or examination of the late consequences of antigen presentation in vivo. However there is increasing recognition that events defined in vitro might not correspond entirely to the physiological situation in vivo. Recent developments in imaging technology now allow realtime observation of single-cell and molecular interactions in intact lymphoid tissues and have already contributed to a more detailed picture of how cells coordinate the initiation or suppression of an immune response. Introduction Until recently the only method of demonstrating antigen processing and peptide-major histocompatibility complex pMHC formation by antigen-presenting cells APCs in vivo was to measure antigen-specific T cell activation in vitro 1 2 . Although these T cell-based assay systems are very sensitive their drawbacks are variations in the stimulatory capacity of different APC populations and the unknown activation state of the responder