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Báo cáo y học: "Immune complexes from rheumatoid arthritis synovial fluid induce FcγRIIa dependent and rheumatoid factor correlated production of tumour necrosis factor-α by peripheral blood mononuclear cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Immune complexes from rheumatoid arthritis synovial fluid induce FcγRIIa dependent and rheumatoid factor correlated production of tumour necrosis factor-α by peripheral blood mononuclear cells. | Available online http arthritis-research.eom content 8 3 R64 Research article Immune complexes from rheumatoid arthritis synovial fluid induce FcYRIIa dependent and rheumatoid factor correlated production of tumour necrosis factor-a by peripheral blood mononuclear cells Linda Mathsson1 Jon Lampa2 Mohammed Mullazehi1 and Johan Ronnelid1 2 1Unit of Clinical Immunology Uppsala University Uppsala Sweden 2Unit of Rheumatology Karolinska Institute Stockholm Sweden Corresponding author Linda Mathsson linda.mathsson@klinimm.uu.se Received 23 Jun 2005 Revisions requested 26 Jul 2005 Revisions received 10 Feb 2006 Accepted 20 Feb 2006 Published 28 Mar 2006 Arthritis Research Therapy 2006 8 R64 doi 10.1186 ar1 926 This article is online at http arthritis-research.com content 8 3 R64 2006 Mathsson et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Immune complexes ICs can induce production of cytokines by peripheral blood mononuclear cells via Fc receptors. Rheumatoid factor RF develop in response to ICs in many clinical and experimental settings. We investigated whether and how polyethylene glycol PEG precipitated ICs from rheumatoid arthritis RA sera and synovial fluid SF can influence cytokine production by peripheral blood mononuclear cells. We also examined the relationship between RF and IC induced cytokine production. Parallel sera and SF from 47 RA patients and sera from 15 healthy control individuals were PEG precipitated. The precipitates were added to serum-free peripheral blood mononuclear cell cultures and tumour necrosis factor TNF -a levels were measured after 20 hours. In separate cell culture experiments FcyRIIa and FcyRIII were blocked and monocytes were depleted or enriched. RF in serum was .