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Báo cáo y học: "Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils. | Available online http arthritis-research.eom content 9 2 R25 Research article Differential expression of RANK RANK-L and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils Patrice E Poubelle Arpita Chakravarti Maria J Fernandes Karine Doiron and Andrée-Anne Marceau Open Access Centre de Recherche en Rhumatologie et Immunologie Centre de Recherche du Centre Hospitalier de l Université Laval CRCHUL 2705 boulevard Laurier Ste-Foy QC G1V 4G2 Canada Corresponding author Patrice E Poubelle Patrice.Poubelle@crchul.ulaval.ca Received 26 Oct 2006 Revisions requested 4 Jan 2007 Revisions received 9 Feb 2007 Accepted 6 Mar 2007 Published 6 Mar 2007 Arthritis Research Therapy 2007 9 R25 doi 10.1186 ar2137 This article is online at http arthritis-research.com content 9 2 R25 2007 Poubelle et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Functional links between bone remodeling and the immune system in chronic inflammatory arthritis are mediated in part by the ligand of receptor activator of nuclear factor-kappa-B RANK-L . Because neutrophils play a crucial role in chronic inflammation the goal of this study was to determine whether proteins of the RANK RANK-L pathway are expressed by synovial fluid SF neutrophils from patients with rheumatoid arthritis RA and to characterize this pathway in normal human blood neutrophils. The expression of RANK-L osteoprotegerin OPG RANK and tumor necrosis factor receptor-associated factor 6 TRAF6 was determined by polymerase chain reaction enzyme-linked immunosorbent assay Western blotting and cytofluorometry. RANK signaling was analyzed by the degradation of inhibitor of kappaB-alpha I-KB-a . SF neutrophils from .