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Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood. | Available online http arthritis-research.eom content 10 2 R37 Research article TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood Qian Zhang1 Ruolin Guo1 Edward M Schwarz2 Brendan F Boyce1 2 and Lianping Xing1 2 Department of Pathology and Laboratory Medicine University of Rochester Medical Center 601 Elmwood Avenue Box 626 Rochester NY 14642 USA 2Center for Musculoskeletal Research University of Rochester Medical Center Rochester NY 14642 USA Corresponding author Lianping Xing lianping_xing@urmc.rochester.edu Received 21 Dec 2007 Revisions requested 8 Feb 2008 Revisions received 14 Mar 2008 Accepted 27 Mar 2008 Published 27 Mar 2008 Arthritis Research Therapy 2008 10 R37 doi 10.1186 ar2391 This article is online at http arthritis-research.com content 10 2 R37 2008 Zhang et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction The objective of the present study was to investigate the role of the stromal cell-derived factor 1 SDF-1 CXCR4 axis in TNF-induced mobilization of osteoclast precursors OCPs from bone marrow. Methods OCPs were generated from bone marrow cells of TNF-transgenic mice or wild-type mice treated with TNF or PBS. The percentage of CD11 b Gr- lo OCPs was assessed by fluorescence-activated cell sorting. OCP migration to the SDF-1 gradient and the osteoclast forming potency were assessed in chemotaxis osteoclastogenic assays. SDF-1 expression was assessed by real-time RT-PCR ELISA and immunostaining in primary bone marrow stromal cells in the ST2 bone marrow stromal cell line and in bones from TNF-injected mice. Results OCPs generated in vitro from wild-type mice .