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Báo cáo y học: "Multi analyte profiling and variability of inflammatory markers in blood and induced sputum in patients with stable COPD"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Multi analyte profiling and variability of inflammatory markers in blood and induced sputum in patients with stable COPD. | Aaron et al. Respiratory Research 2010 11 41 http respiratory-research.eom content 11 1 41 RESPIRATORY RESEARCH RESEARCH Open Access Multi analyte profiling and variability of inflammatory markers in blood and induced sputum in patients with stable COPD Shawn D Aaron 1 Katherine L Vandemheen1 Timothy Ramsay1 Chun Zhang2 Zafrira Avnur2 Tania Nikolcheva2 and Anthony Quinn2 Abstract Background We analyzed serial concentrations of multiple inflammatory mediators from serum and induced sputum obtained from patients with stable COPD and controls. The objective was to determine which proteins could be used as reliable biomarkers to assess COPD disease state and severity. Methods Forty-two subjects 21 with stable COPD and 21 controls were studied every 2 weeks over a 6-week period. Serum and induced sputum were obtained at each of 3 visits and concentrations of 19 serum and 22 sputum proteins were serially assessed using multiplex immunoassays. We used linear mixed effects models to test the distribution of proteins for an association with COPD and disease severity. Measures of within- and between-subject coefficients of variation were calculated for each of the proteins to assess reliability of measurement. Results There was significant variability in concentrations of all inflammatory proteins over time and variability was greater for sputum proteins median intra-subject coefficient of variation 0.58 compared to proteins measured in serum median intra-subject coefficient of variation 0.32 P 0.03 . Of 19 serum proteins and 22 sputum proteins tested only serum CRP myeloperoxidase and VEGF and sputum IL-6 IL-8 TIMP-1 and VEGF showed acceptable intra and inter-patient reliability and were significantly associated with COPD the severity of lung function impairment and dyspnea. Conclusions Levels of many serum and sputum biomarkers cannot be reliably ascertained based on single measurements. Multiple measurements over time can give a more reliable and precise estimate of the .