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Báo cáo y học: " Expression profiling identifies genes involved in emphysema severity"

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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Expression profiling identifies genes involved in emphysema severity. | Respiratory Research BioMed Central Open Access Expression profiling identifies genes involved in emphysema severity Santiyagu M Savarimuthu Francis 1 2 Jill E Larsen1 2 Sandra J Pavey2 Rayleen V Bowman1 2 Nicholas K Hayward2 3 Kwun M Fong1 2 and Ian A Yang1 2 Address Department of Thoracic Medicine The Prince Charles Hospital Brisbane Australia 2School of Medicine The University of Queensland Brisbane Australia and 3Department of human genetics Oncogenomics Laboratory Queensland Institute of Medical Research Brisbane Australia Email Santiyagu M Savarimuthu Francis - ss.francis@uqconnect.edu.au Jill E Larsen - j.larsen@uq.edu.au Sandra J Pavey - s.pavey1@uq.edu.au Rayleen V Bowman - rayleen_bowman@health.qld.gov.au Nicholas K Hayward - Nick.Hayward@qimr.edu.au Kwun M Fong - Kwun_Fong@health.qld.gov.au Ian A Yang - Ian_Yang@health.qld.gov.au Corresponding author Published 2 September 2009 Received 10 May 2009 Respiratory Research 2009 10 81 doi l0.ll86 l465-992l-l0-8l Accepted 2 September 2009 This article is available from http respiratory-research.cOm content l0 l 8l 2009 Francis et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Chronic obstructive pulmonary disease COPD is a major public health problem. The aim of this study was to identify genes involved in emphysema severity in COPD patients. Gene expression profiling was performed on total RNA extracted from non-tumor lung tissue from 30 smokers with emphysema. Class comparison analysis based on gas transfer measurement was performed to identify differentially expressed genes. Genes were then selected for technical validation by quantitative reverse transcriptase-PCR qRT-PCR if also represented on microarray platforms used in previously published emphysema

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