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Báo cáo y học: "Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor (SLPI) by lung epithelial cells: role of charge of the proteinase-inhibitor complex"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor (SLPI) by lung epithelial cells: role of charge of the proteinase-inhibitor complex. | Respiratory Research BioMed Central Open Access Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor SLPI by lung epithelial cells role of charge of the proteinase-inhibitor complex Anita L Sullivan1 Timothy Dafforn2 Pieter S Hiemstra3 and Robert A Stockley 4 Address Department of Medicine University of Birmingham UK 2Department of Biosciences University of Birmingham UK 3Department of Pulmonology Leiden University Medical Centre The Netherlands and 4Department of Respiratory Medicine University Hospitals Birmingham Birmingham UK Email Anita L Sullivan - a.l.sullivan@bham.ac.uk Timothy Dafforn - t.r.dafforn@bham.ac.uk Pieter S Hiemstra - P.S.Hiemstra@lumc.nl Robert A Stockley - r.a.stockley@bham.ac.uk Corresponding author Published 12 August 2008 Received 28 January 2008 Respiratory Research 2008 9 60 doi l0.ll86 l465-992l-9-60 Accepted 12 August 2008 This article is available from http respiratory-research.cOm content 9 l 60 2008 Sullivan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Secretory leukoproteinase inhibitor SLPI is an important inhibitor of neutrophil elastase NE a proteinase implicated in the pathogenesis of lung diseases such as COPD. SLPI also has antimicrobial and anti-inflammatory properties but the concentration of SLPI in lung secretions in COPD varies inversely with infection and the concentration of NE. A fall in SLPI concentration is also seen in culture supernatants of respiratory cells exposed to NE for unknown reasons. We investigated the hypothesis that SLPI complexed with NE associates with cell membranes in vitro. Methods Respiratory epithelial cells were cultured