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Báo cáo y học: "The involvement of interleukin-1 and interleukin-4 in the response of human annulus fibrosus cells to cyclic tensile strain: an altered mechanotransduction pathway with degeneration"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The involvement of interleukin-1 and interleukin-4 in the response of human annulus fibrosus cells to cyclic tensile strain: an altered mechanotransduction pathway with degeneration. | Gilbert et al. Arthritis Research Therapy 2011 13 R8 http arthritis-research.eom content 13 1 R8 RESEARCH ARTICLE Open Access The involvement of interleukin-1 and interleukin-4 in the response of human annulus fibrosus cells to cyclic tensile strain an altered mechanotransduction pathway with degeneration Hamish TJ Gilbert Judith A Hoyland Anthony J Freemont Sarah J Millward-Sadler Abstract Introduction Recent evidence suggests that intervertebral disc IVD cells derived from degenerative tissue are unable to respond to physiologically relevant mechanical stimuli in the normal anabolic manner but instead respond by increasing matrix catabolism. Understanding the nature of the biological processes which allow disc cells to sense and respond to mechanical stimuli a process termed mechanotransduction is important to ascertain whether these signalling pathways differ with disease. The aim here was to investigate the involvement of interleukin IL -1 and IL-4 in the response of annulus fibrosus AF cells derived from nondegenerative and degenerative tissue to cyclic tensile strain to determine whether cytokine involvement differed with IVD degeneration. Methods AF cells were isolated from nondegenerative and degenerative human IVDs expanded in monolayers and cyclically strained in the presence or absence of the cytokine inhibitors IL-1 receptor antagonist IL-1Ra or IL-4 receptor antibody IL-4RAb with 10 strain at 1.0 Hz for 20 minutes using a Flexcell strain device. Total RNA was extracted from the cells at time points of baseline control and 1 or 24 hours poststimulation. Quantitative real-time polymerase chain reaction was used to analyse the gene expression of matrix proteins aggrecan and type I collagen and enzymes matrix metalloproteinase 3 MMP3 and a disintegrin and metalloproteinase with a thrombospondin type 1 motif 4 ADAMTS4 . Results Expression of catabolic genes MMP3 and ADAMTS4 decreased in AF cells derived from nondegenerative tissue in response to 1.0-Hz .