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Báo cáo y học: " Induction and regulation of matrix metalloproteinase-12in human airway smooth muscle cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Induction and regulation of matrix metalloproteinase-12in human airway smooth muscle cells. | Respiratory Research BioMed Central Research Open Access Induction and regulation of matrix metalloproteinase-l2in human airway smooth muscle cells Shaoping Xie1 Razao Issa1 Maria B Sukkar1 Ute Oltmanns1 Pankaj K Bhavsar1 Alberto Papi2 Gaetano Caramori2 Ian Adcock1 and K Fan Chung 1 Address Experimental Studies National Heart and Lung Institute Imperial College London UK and 2Centro di Ricerca su Asma e BPCO University of Ferrara Ferrara Italy Email ShaopingXie - s.xie@imperial.ac.uk Razao Issa - razao.issa@imperial.ac.uk Maria B Sukkar - m.sukkar@imperial.ac.uk Ute Oltmanns - u.oltmanns@imperial.ac.uk PankajK Bhavsar - p.bhavsar@ic.ac.uk Alberto Papi - ppa@unife.it Gaetano Caramori - crm@unife.it Ian Adcock - ian.adcock@ic.ac.uk K Fan Chung - f.chung@imperial.ac.uk Corresponding author Published 16 December 2005 Received 01 April 2005 Respiratory Research 2005 6 148 doi 10.1186 1465-9921-6-148 Accepted 16 December 2005 This article is available from http respiratory-research.cOm content 6 1 148 2005 Xie et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The elastolytic enzyme matrix metalloproteinase MMP -12 has been implicated in the development of airway inflammation and remodeling. We investigated whether human airway smooth muscle cells could express and secrete MMP-12 thereby participating in the pathogenesis of airway inflammatory diseases. Methods Laser capture microdissection was used to collect smooth muscle cells from human bronchial biopsy sections. MMP-12 mRNA expression was analysed by quantitative real-time RT-PCR. MMP-12 protein expression and secretion from cultured primary airway smooth muscle cells was further analysed by Western blot. MMP-12 protein localization in bronchial tissue