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Báo cáo khoa học: " Biochemical responses and oxidative stress in Francisella tularensis infection: a European brown hare model"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Biochemical responses and oxidative stress in Francisella tularensis infection: a European brown hare model. | Bandouchova et al. Acta Veterinaria Scandinavica 2011 53 2 http www.actavetscand.eom content 53 1 2 AVS ACTAVETERINARIA tTndTXZ 0 SCANDINAVICA RESEARCH Open Access Biochemical responses and oxidative stress in Francisella tularensis infection a European brown hare model 1 f 2f 2.3 1 1 Hana Bandouchova Miroslav Pohanka Kristina Vlckova Veronika Damkova Lucie Peckova Jana Sedlackova1 Frantisek Treml4f Frantisek Vitula1 Jiri Pikula1 f Abstract Background The aim of the present study was to investigate biochemical and oxidative stress responses to experimental F. tularensis infection in European brown hares an important source of human tularemia infections. Methods For these purposes we compared the development of an array of biochemical parameters measured in blood plasma using standard procedures of dry chemistry as well as electrochemical devices following a subcutaneous infection with a wild Francisella tularensis subsp. holarctica strain a single dose of 2.6 X 109 CFU pro toto . Results Subcutaneous inoculation of a single dose with 2.6 X 109 colony forming units of a wild F. tularensis strain pro toto resulted in the death of two out of five hares. Plasma chemistry profiles were examined on days 2 to 35 post-infection. When compared to controls the total protein urea lactate dehydrogenase aspartate aminotransferase and alanine aminotransferase were increased while albumin glucose and amylase were decreased. Both uric and ascorbic acids and glutathione dropped on day 2 and then increased significantly on days 6 to 12 and 6 to 14 post-inoculation respectively. There was a two-fold increase in lipid peroxidation on days 4 to 8 post-inoculation. Conclusions Contrary to all expectations the present study demonstrates that the European brown hare shows relatively low susceptibility to tularemia. Therefore the circumstances of tularemia in hares under natural conditions should be further studied. Background Tularemia is considered a re-emerging zoonosis 1-3 that is .