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Báo cáo khoa học: " Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes. | Nishita et al. Acta Veterinaria Scandinavica 2011 53 16 http www.actavetscand.eom content 53 1 16 AVS ACTAVETERINARIA SCANDINAVICA RESEARCH Open Access Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes Tí chihrs l lichlc.1 1V IiTcsnoipo2 Imonori1 Ipci mo li bibom3 @iriFc 4 071 ixmcbi Aricbimo5 losililio Nisilitd ĩuicimo lomitd Idkdo Illldlldll Nobutsune ICIIIIIdld Kensuke orito Kdzuyosin AnsilHIld Abstract Background Cdrbonic dnhydrdse CA of the chicken hds dttrdcted dttention for d long time becduse it hds dn importdnt role in the eggshell formdtion. The developmentdl profile of CA-II isozyme levels in chicken erythrocytes hds not been determined or reported. Furthermore the reldtions with CA-II in erythrocyte dnd egg production dre not discussed. In the present study we isoldted CA-II from erythrocytes of chickens dnd determined dge-reldted chdnges of CA-II levels in erythrocytes. Methods Chicken CA-II wds purified by d combindtion of column chromdtogrdphy. The levels of CA-II in the hemolysdte of the chicken were determined using the ELISA system in blood sdmples from 279 femdle chickens dges 1 to 93 weeks 69 mdle chickens dges 3 to 59 weeks dnd 52 weeks femdle Arducdnd-chickens. Results The medn concentration of CA-II in hemolysdte from 1-week-old femdle wds 50.8 11.9 mg g of Hb. The medn levels of CA-II in 25-week-old 188.1 82.6 mg g of Hb 31-week-old 193.6 69.7 mg g of Hb dnd 49-week-old 203.8 123.5 mg g of Hb femdle-chickens showed the highest level of CA-II. The levels of CA-II in femdle WL-chickens significdntly decredsed dt 63 week 139.0 19.3 mg g of Hb . The levels of CA-II in femdle WL-chicken did not chdnge from week 63 until week 93.The medn level of CA-II in hemolysdte of 3-week-old mdle WL-chickens wds 78.3 20.7 mg g of Hb. The levels of CA-II in mdle WL-chickens did not show chdnges in the week 3 to week 59 timeframe. The medn level of CA-II in 53-week-old femdle Arducdnd-chickens wds 23.4 1.78 mg g .