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Báo cáo y học: "Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo. | Retrovirology BioMed Central Research Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4 T lymphocytes in human lymphoid tissue ex vivo Stefanie Homann1 Nadine Tibroni1 Ingo Baumann2 Serkan Sertel2 Oliver T Keppler1 and Oliver T Fackler 1 Open Access Address Department of Virology University of Heidelberg Heidelberg Germany and 2Department of Otolaryngology Head and Neck Surgery University of Heidelberg Heidelberg Germany Email Stefanie Homann - Stefanie.Homann@med.uni-heidelberg.de Nadine Tibroni - Nadine.Tibroni@med.uni-heidelberg.de Ingo Baumann - Ingo.Baumann@med.uni-heidelberg.de Serkan Sertel - Serkan.Sertel@med.uni-heidelberg.de Oliver T Keppler - Oliver.Keppler@med.uni-heidelberg.de Oliver T Fackler - Oliver.Fackler@med.uni-heidelberg.de Corresponding author Published 15 January 2009 Received 6 November 2008 Accepted 15 January 2009 Retrovirology 2009 6 6 doi l0.ll86 l742-4690-6-6 This article is available from http www.retrovirology.cOm content 6 1 6 2009 Homann et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background HIV-1 Nef critically contributes to AIDS in part by augmenting virus titers in infected individuals. Analyzing which of Nefs activities contribute to HIV pathogenesis has been hampered by the lack of a cell culture model in which Nef exerts pronounced effects on HIV replication. The human lymphoid aggregate culture HLAC from tonsil maintains the cell populations and cytokine milieu found in vivo supports a productive infection without exogenous stimulation and Nef contributes to efficient HIV-1 replication as well as CD4 T cell depletion in this experimental ex vivo-model. Results To identify determinants in Nef that mediate these activities we .