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DNA Methylation: Basic Mechanisms - Part 6

Phương pháp của methyl hóa phân tích nhạy cảm với sự khác biệt đại diện (MS-RDA) là dựa trên một giao thức lai trừ sau khi chọn chống lại các mảnh DNA được rất nhiều methyl hóa và do đó, không bị phân cắt bởi các hạn chế endonuclease HpaII (Ushijima et al 1997). | 160 w. Doerfler The mefood of mefoylation-sensitive representational difference analysis MS-RDA is based upon a subtractive hybridization protocol after selecting against DNA fragments that were heavily methylated and therefore not cleaved by the Hpall restriction endonuclease Ushijima et al. 1997 . Weop-plied this mudrodto lheieveiirganon oft.ranscripl.s 7rom Oneieriophage th DNA-transgnntchamster Citi licae in eompaslseatohamatep cell hnesdenaih of integr i hiO n DNA iMullerei tai. soorg -h using thasupaeessive hybridization lechmahe hsa ma i y i is nf complementer. eiDNA preparstiohh rtti r non-lrannnediCiAd1hDNAP.ransgenicosid0. lSNA-t.oao1geokPamslet ce is several coClutes genee wot dunedthathad tdtered prnnseriptìontd ì c iDo- in the trantgsnic an compared lothe non-Cransgemceells. Amonr indivtdual non-tran1oenicnam1ter cdldontr inveellgated as nwgotioe C tniro-1 no difl ferences in hDNAiri i90es ond hencr rran7eeiptionatptd-hes ward gbssoncV. We also siadied foeae dlg-es in oeeẰ DNA-ttan9nemcmonsestrcio Hy-permethn-atienwaseogndSor si- -mprintedeoane gene for DNA Orom heari muscle. Twomoust lintt rraocgenicfo7 nnAS2 promoles--ndigdror i-enpcon-struct shewee hypomt7hyiat.iog D the totselewkóc- 9nd 1 27 peonr Wi concludei-iba-m Adss DNA- re h DNdtieansggnic Sien í i Ế r cells OTmict cd-lular mePnlation pnd tS9nscripSlodnatttnns ran de ei it op altered MtiHei et al. 200 li Detailedmnestìgatthn1 on foe 0ntruodenoily oli ien -ronse9ipi.iod patterns in about l 9i0 j t mOT gmt i h iSi drMi ot BHK21 andT637cpllh hine revealed only mtnimal differences in 5 of these genes by DNA array analyses between thotwo dS o r s and omongdifSenent. clone s co tach edlline -N. Hochstem 9hO w. Datrnet nnppOiisheh oxpeiimtntal. Since iPeinser i.top pf forcignDlnAigta e1tabhshenmommslianncnomes has htcome P nrefersePseg-men-n expe9imtntalbiaiony e.-gin ihen-nerar tion of traosgenic oiganismo and inciegsihgly olsoivoepetherapy I consider it an important problemto potsoc tic .