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Báo cáo y học: " Effect of chloroquine on reducing HIV-1 replication in vitro and the DC-SIGN mediated transfer of virus to CD4+ T-lymphocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài:" Effect of chloroquine on reducing HIV-1 replication in vitro and the DC-SIGN mediated transfer of virus to CD4+ T-lymphocytes. | Retrovirology BioMed Central Research Effect of chloroquine on reducing HIV-I replication in vitro and the DC-SIGN mediated transfer of virus to CD4 T-lymphocytes Marloes A Naarding Elly Baan Georgios Pollakis and William A Paxton Open Access Address Laboratory of Experimental Virology Department of Medical Microbiology Center for Infection and Immunity Amsterdam CINIMA Academic Medical Center University of Amsterdam Meibergdreef 15 1105 AZ Amsterdam The Netherlands Email Marloes A Naarding - m.a.naarding@amc.uva.nl Elly Baan - e.baan@amc.uva.nl Georgios Pollakis - g.pollakis@amc.uva.nl William A Paxton - w.a.paxton@amc.uva.nl Corresponding author Published 30 January 2007 Received I December 2006 Accepted 30 January 2007 Retrovirology 2007 4 6 doi 10.1186 1742-4690-4-6 p This article is available from http www.retrovirology.com content 4 I 6 2007 Naarding et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Chloroquine CQ has been shown to inhibit HIV-1 replication in vitro as well as in vivo and has been proposed to alter the glycosylation pattern of the gp 1 20 envelope. These activities indicate that the compound can be used not only as an effective HIV-1 therapeutic agent but also as a modulator of the gp120 envelope protein structure enabling for the production of broader neutralizing Ab responses. Results We confirm here that HIV-1 replication on CD4 T-lymphocytes can be reduced in the presence of CQ and show that the reduced replication is producer cell mediated with viruses generated in the presence of CQ not being inhibited for subsequent infectivity and replication. By analysing the gp120 envelope protein sequences from viruses cultured long-term in the absence or presence of CQ we .