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Báo cáo y học: "Membrane transporters and protein traffic networks differentially affecting metal tolerance: a genomic phenotyping study in yeas"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Membrane transporters and protein traffic networks differentially affecting metal tolerance: a genomic phenotyping study in yeast. | Research Open Access Membrane transporters and protein traffic networks differentially affecting metal tolerance a genomic phenotyping study in yeast Roberta Ruotolo Gessica Marchini and Simone Ottonello Address Department of Biochemistry and Molecular Biology Viale G.P. Usberti 23 A University of Parma I-43100 Parma Italy. Correspondence Simone Ottonello. Email s.ottonello@unipr.it Published 7 April 2008 Genome Biology 2008 9 R67 doi 10.1 186 gb-2008-9-4-r67 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2008 9 4 R67 Received 29 December 2007 Revised 26 February 2008 Accepted 7 April 2008 2008 Ruotolo et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The cellular mechanisms that underlie metal toxicity and detoxification are rather variegated and incompletely understood. Genomic phenotyping was used to assess the roles played by all nonessential Saccharomyces cerevisiae proteins in modulating cell viability after exposure to cadmium nickel and other metals. Results A number of novel genes and pathways that affect multimetal as well as metal-specific tolerance were discovered. Although the vacuole emerged as a major hot spot for metal detoxification we also identified a number of pathways that play a more general less direct role in promoting cell survival under stress conditions for example mRNA decay nucleocytoplasmic transport and iron acquisition as well as proteins that are more proximally related to metal damage prevention or repair. Most prominent among the latter are various nutrient transporters previously not associated with metal toxicity. A strikingly differential effect was observed for a large set of deletions the majority .