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Báo cáo sinh học: "Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học quốc tế đề tài: Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score | Brand et al. Genetics Selection Evolution 2011 43 24 http www.gsejournal.Org content 43 1 24 GSE Ge n et i cs Selection Evolution RESEARCH Open Access Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score o J D m vs Z 1 1 A I I_I s vt m s 1 IH I v v D V r I I p.z r2 D z- -f-f- I k- - f . ri z r PkZ r lz ĩ I I r pi4 i I A z 1llk- 1i f--75 Pk r I r t s IXi I l v 3 Bodo Diana Anja Hartmann Dirk Repsilber Denina GriesoecK-ziicn Olga wellnitz Christa KUhn Siriluck Ponsuksili1 Heinrich HD Meyer4 and Manfred Schwerin1 6 Abstract Background During the past ten years many quantitative trait loci QTL affecting mastitis incidence and mastitis related traits like somatic cell score SCS were identified in cattle. However little is Known about the molecular architecture of QTL affecting mastitis susceptibility and the underlying physiological mechanisms and genes causing mastitis susceptibility. Here a genome-wide expression analysis was conducted to analyze molecular mechanisms of mastitis susceptibility that are affected by a specific QTL for SCS on Bos taurus autosome 18 BTA18 . Thereby some first insights were sought into the genetically determined mechanisms of mammary gland epithelial cells influencing the course of infection. Methods Primary bovine mammary gland epithelial cells pbMEC were sampled from the udder parenchyma of cows selected for high and low mastitis susceptibility by applying a marker-assisted selection strategy considering QTL and molecular marker information of a confirmed QTL for SCS in the telomeric region of BTA18. The cells were cultured and subsequently inoculated with heat-inactivated mastitis pathogens Escherichia coli and Staphylococcus aureus respectively. After 1 6 and 24 h the cells were harvested and analyzed using the microarray expression chip technology to identify differences in mRNA expression profiles attributed to