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Báo cáo sinh học: "Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes | Genet. Sel. Evol. 40 2008 215-226 INRA EDP Sciences 2008 DOI 10.1051 gse 2007045 Available online at www.gse-journal.org Original article Cloning chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes Xia Tao Yuan JiHONG Gan Li Feng BIN Zhu Yi Chen XiAODONG Zhang PEICHAO Zaiqing Yang Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 P. R. China Received 4 December 2006 accepted 31 August 2007 Abstract - The PAT proteins named after the three PLIN ADRP TIP47 PAT proteins PLIN for perilipin ADRP for adipose differentiation-related protein and TIP47 for tail-interacting protein of 47 kDa now officially named M6PRBP1 for mannose-6-phosphate receptor binding protein 1 is a set of intracellular lipid droplet binding proteins. They are localized in the outer membrane monolayer enveloping lipid droplets and are involved in the metabolism of intracellular lipid. This work describes the cloning and sequencing of porcine PLIN and M6PRBP1 cDNAs the chromosome mapping of these two genes as well as the expression pattern of porcine PAT genes. Sequence analysis shows that the porcine PLIN cDNA contains an open reading frame of 1551 bp encoding 516 amino acids and that the porcine M6PRBP1 cDNA contains a coding region of 1320 bp encoding 439 amino acids. Comparison of PLIN and M6PRBP1 amino-acid sequences among various species reveals that porcine and bovine proteins are the most conserved. Porcine PLIN and M6PRBP1 genes have been mapped to pig chromosomes 7 and 2 respectively by radiation hybrid analysis using the IMpRH panel. Expression analyses in pig showed a high expression of PLIN mRNA in adipose tissue M6PRBP1 mRNA in small intestine kidney and spleen and ADRP mRNA in adipose tissue lung and spleen. pig PLIN M6PRBP1 cDNA cloning chromosome mapping tissue expression pattern 1. INTRODUCTION Intracellular neutral lipid .