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Báo cáo sinh học: "DNA vaccine constructs against enterovirus 71 elicit immune response in mice"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: DNA vaccine constructs against enterovirus 71 elicit immune response in mice | Genetic Vaccines and Therapy BioMed Central Research DNA vaccine constructs against enterovirus 71 elicit immune response in mice Wong SiewTung1 Sazaly Abu Bakar2 Zamberi Sekawi3 and Rozita Rosli 1 Open Access Address 1Dept. of Human Growth and Development Faculty of Medicine and Health Sciences Universiti Putra Malaysia 43400 Serdang Selangor Malaysia 2Department of Medical Microbiology Faculty of Medicine Universiti Malaya Medical Center 50603 Kuala Lumpur Malaysia and 3Dept of Clinical Laboratory Sciences Faculty of Medicine and Health Sciences Universiti Putra Malaysia 43400 Serdang Selangor Malaysia Email Wong SiewTung - siewtung77@hotmail.com Sazaly Abu Bakar - sazaly@ummc.edu.my Zamberi Sekawi - zamberi@medic.upm.edu.my Rozita Rosli - rozita@medic.upm.edu.my Corresponding author Published 19 April 2007 Received 5 September 2006 _ r JIA I nrr r Accepted 19 April 2007 Genetic Vaccines and Therapy 2007 5 6 doi 10.1186 1479-0556-5-6 This article is available from http www.gvt-journal.cOm content 5 1 6 2007 Tung et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background Enterovirus 71 EV71 is a major causative viral agent responsible for large outbreaks of hand foot and mouth disease HFMD a common rash illness in children and infants. There is no effective antiviral treatment for severe EV71 infections and no vaccine is available. The objectives of this study were to design and construct a DNA vaccine against Enterovirus 71 using the viral capsid protein VP1 gene of EV71 and to verify the functionality of the DNA vaccine in vitro and in vivo. Methods The VP1 gene of EV71 from two local outbreak isolates were amplified using PCR