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Báo cáo sinh học: "Therapeutic dendritic cell vaccine preparation using tumor RNA transfection: A promising approach for the treatment of prostate cancer"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Therapeutic dendritic cell vaccine preparation using tumor RNA transfection: A promising approach for the treatment of prostate cancer | Genetic Vaccines and Therapy BioMed Central Research Open Access Therapeutic dendritic cell vaccine preparation using tumor RNA transfection A promising approach for the treatment of prostate cancer Juliana M Sousa-Canavez 1 Flavio C Canavez1 Kátia RM Leite1 2 and Luiz H Camara-Lopes1 Address 1Oncocell Division Genoa Biotechnology SA Alameda Ministro Rocha Azevedo 346 1st floor 01410-000 São Paulo SP Brazil and 2Laboratório de Investigaẹão Médica da Disciplina de Urologia da Faculdade de Medicina de USP-LIM 55 São Paulo SP Brazil Email Juliana M Sousa-Canavez - jmscanavez@genoabiotec.com.br Flavio C Canavez - fccanavez@genoabiotec.com.br Kátia RM Leite - katiaramos@uol.com.br Luiz H Camara-Lopes - lhcamaralopes@uol.com.br Corresponding author Published 18 January 2008 Received 22 October 2007 Genetic Vaccines and Therapy 2008 6 2 doi l0.ll86 l479-0556-6-2 Accepted 18 January 2008 This article is available from http www.gvt-journal.cOm content 6 1 2 2008 Sousa-Canavez et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Early prostate adenocarcinoma can be diagnosed through seric prostate-specific antigen PSA screenings. However a fraction of patients progress to an incurable metastatic disease. Therefore novel therapies for treating these patients are extremely desirable. Therapeutic vaccines based on Dendritic Cells DCs carrying tumor antigens have emerged as a promising strategy to initiate an immune response against tumor cells. These vaccines can be prepared using different methodologies such as the application of tumor mRNA described in this work. Methods Mature and immature DCs were obtained in vitro by adding specific cytokines to monocyte cell cultures. RNA extracted from prostate tumor lineage