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Báo cáo khoa hoc:" Genetic variation in two conserved local Romanian pig breeds using type 1 DNA markers"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Genetic variation in two conserved local Romanian pig breeds using type 1 DNA markers | Genet Sei. Evol. 33 2001 417-432 417 INRA EDP Sciences 2001 Original article Genetic variation in two conserved local Romanian pig breeds using type 1 DNA markers Daniel c. Ciobanua b Andrew E. Dayc Alexandru Nagyd Richard Walesc Max F. Rothschildb Graham s. Plastowc a Animal Genetics Unit University of Agricultural Sciences and Veterinary Medicine 3400 Cluj Napoca Romania b Department of Animal Science 2255 Kildee Hall Iowa State University Ames IA 50011 USA c PIC International Group Fyfield Wick OX13 5NA UK d Agricultural Research Station of Turda Romania Received 7 August 2000 accepted 20 February 2001 Abstract - Analysis of the genetic variation of an endangered population is an important component for the success of conservation. Animals from two local Romanian pig breeds the Mangalitsa and Bazna were analysed for variation at a number of genetic loci using PCR-based DNA tests. Polymorphism was assessed at loci which 1 are known to cause phenotypic variation 2 are potentially involved in trait differences or 3 are putative candidate genes. The traits considered are disease resistance growth coat colour meat quality and prolificacy. Even though the populations are small and the markers are limited to specific genes we found significant differences in five of the ten characterised loci. In some cases the observed allele frequencies were interesting in relation to gene function and the phenotype of the breed. These breeds are part of a conservation programme in Romania and marker information may be useful in preserving a representative gene pool in the populations. The use of polymorphisms in type 1 gene markers may be a useful complement to analysis based on anonymous markers. pig genetic diversity local breeds 1. introduction Domestic animals have been an important element of human development satisfying needs for food clothing and power. About 40 different species have been used and humans have produced some 4 500 breeds known today as the world s animal .