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Báo cáo sinh học: Chromosomal localization and activity of nucleolar organizer regions in the dog

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Chromosomal localization and activity of nucleolar organizer regions in the dog | 79 Genet. Sei. Evol. 30 1998 79-84 Inra Elsevier Paris Note Chromosomal localization and activity of nucleolar organizer regions in the dog Cam s familiaris Aldona Pienkowska Marek Switonski Department of Genetics and Animal Breeding Agricultural University of Poznan Wolynska 33 60-637 Poznan Poland Received 2 June 1997 accepted 5 December 1997 Abstract - Using fluorescent in situ hybridization on Q-banded chromosomes nucleolar organizer regions NORs were localized on canine chromosomes 7qter 17qter Yqter and also terminally on a small autosome not yet included in the dog standard karyotype. The analysis of NOR activity was carried out on Ag-I stained metaphases originating from 27 individuals 10 males and 17 females . The mean number of silver stained NORs was 6.0 in males and 5.3 in females and the modal number was 7 and 6 respectively. Interindividual variability of the mean and modal numbers of silver NORs was observed. The lowest individual mean number of NORs was 4.2 and the highest 7.0 and the modal value ranged from 4 to 7. Inra Elsevier Paris NOR dog fluorescent in situ hybridization I silver staining Resume Localisation chromosomique et activité d organisateurs nucléolaires chez le Chien Cam s familiaris . Les organisateurs nucléolaires NORs des chromosomes visualises en bandes Q ont été localises par hybridation fluorescente in situ sur les chromosomes canins 7qter 17qter Yqter et ainsi qu à 1 extrémité d un petit autosome qui n est pas encore inclus dans le caryotype standard du chien. L activite des organisateurs nucléolaires a été raise en evidence sur des metaphases colorées par l Ag-I provenant de 27 individus 10 males et 17 femelles . Il a été trouvé que le nombre moyen d organisateurs nucléolaires colorés par 1 argent était de 6 chez les mâles et de 5 3 chez les femelies et que la valeur modale était respectivement 7 et 6. Une variabilité interindividuelle du nombre moyen et de la valeur modale a été observée pour les organisateurs nucléolaires .