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Báo cáo y học: "Altered protein expression in serum from endometrial hyperplasia and carcinoma patients"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Altered protein expression in serum from endometrial hyperplasia and carcinoma patients. | Wang et al. Journal of Hematology Oncology 2011 4 15 http www.jhoonline.Org content 4 1 15 JOURNAL OF HEMATOLOGY ONCOLOGY SHORT REPORT Open Access Altered protein expression in serum from endometrial hyperplasia and carcinoma patients Yi-sheng Wang1 Rui Cao2 Hong Jin3 4 Yi-ping Huang1 Xiao-yan Zhang1 Qing Cong1 Yi-feng He1 and Cong-jian Xu1 3 5 6 Abstract Background Endometrial carcinoma is one of the most common gynecological malignancies in women. The diagnosis of the disease at early or premalignant stages is crucial for the patient s prognosis. To date diagnosis and follow-up of endometrial carcinoma and hyperplasia require invasive procedures. Therefore there is considerable demand for the identification of biomarkers to allow non-invasive detection of these conditions. Methods In this study we performed a quantitative proteomics analysis on serum samples from simple endometrial hyperplasia complex endometrial hyperplasia atypical endometrial hyperplasia and endometrial carcinoma patients as well as healthy women. Serum samples were first depleted of high-abundance proteins labeled with isobaric tags iTRAQ and then analyzed via two-dimensional liquid chromatography and tandem mass spectrometry. Protein identification and quantitation information were acquired by comparing the mass spectrometry data against the International Protein Index Database using ProteinPilot software. Bioinformatics annotation of identified proteins was performed by searching against the PANTHER database. Results In total 74 proteins were identified and quantified in serum samples from endometrial lesion patients and healthy women. Using a 1.6-fold change as the benchmark 12 proteins showed significantly altered expression levels in at least one disease group compared with healthy women. Among them 7 proteins were found for the first time to be differentially expressed in atypical endometrial hyperplasia. These proteins are orosomucoid 1 haptoglobin SERPINC 1 alpha-1-antichymotrypsin .