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Báo cáo khoa hoc:" Generation of a panel of antibodies against proteins encoded on human chromosome 21"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Generation of a panel of antibodies against proteins encoded on human chromosome 21 | Wiseman et al. Journal of Negative Results in BioMedicine 2010 9 7 http www.jnrbm.eom content 9 1 7 r M 1 JOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE RESEARCH Open Access Generation of a panel of antibodies against proteins encoded on human chromosome 21 Frances K Wiseman1 Olivia Sheppard1 Jacqueline M Linehan1 Sebastian Brandner1 Victor LJ Tybulewicz2 Elizabeth MC Fisher1 Abstract Background Down syndrome DS is caused by trisomy of all or part of chromosome 21. To further understanding of DS we are working with a mouse model the Tc1 mouse which carries most of human chromosome 21 in addition to the normal mouse chromosome complement. This mouse is a model for human DS and recapitulates many of the features of the human syndrome such as specific heart defects and cerebellar neuronal loss. The Tc1 mouse is mosaic for the human chromosome such that not all cells in the model carry it. Thus to help our investigations we aimed to develop a method to identify cells that carry human chromosome 21 in the Tc1 mouse. To this end we have generated a panel of antibodies raised against proteins encoded by genes on human chromosome 21 that are known to be expressed in the adult brain of Tc1 mice Results We attempted to generate human specific antibodies against proteins encoded by human chromosome 21. We selected proteins that are expressed in the adult brain of Tc1 mice and contain regions of moderate low homology with the mouse ortholog. We produced antibodies to seven human chromosome 21 encoded proteins. Of these we successfully generated three antibodies that preferentially recognise human compared with mouse SOD1 and RRP1 proteins on western blots. However these antibodies did not specifically label cells which carry a freely segregating copy of Hsa21 in the brains of our Tc1 mouse model of DS. Conclusions Although we have successfully isolated new antibodies to SOD1 and RRP1 for use on western blots in our hands these antibodies have not been successfully used for .