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Báo cáo y học: "Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors"

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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors. | Journal of Immune Based Therapies and Vaccines Original research BioMed Central Open Access Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors Smita A Ghanekar 1 Sonny Bhatia1 Joyce J Ruitenberg1 Corazon DeLa Rosa2 Mary L Disis2 Vernon C Maino1 Holden T Maecker1 and Cory A Waters1 Address 1BD Biosciences Immunocytometry Systems 2350 Qume Dr. San Jose CA 95131 USA and 2University of Washington Division of Oncology 815 Mercer St. Seattle Wa 98109 UsA Email Smita A Ghanekar - smita_ghanekar@bd.com Sonny Bhatia - sonny_bhatia@bd.com Joyce J Ruitenberg - joyce_ruitenberg@bd.com Corazon DeLa Rosa - meannie@u.washington.edu Mary L Disis - ndisis@u.washington.edu Vernon C Maino - smaino@bd.com HoldenTMaecker-holden_maecker@bd.com Cory A Waters - cory_waters@bd.com Corresponding author Published 3 May 2007 Received 2 January 2007 Journal of Immune Based Therapies and Vaccines 2007 5 7 doi l0.ll86 l 476-8518-5-7 Accepted 3 May 2007 This article is available from http www.jibtherapies.cOm content 5 1 7 2007 Ghanekar et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Monocyte-derived-dendritic-cells MDDC are the major DC type used in vaccinebased clinical studies for a variety of cancers. In order to assess whether in vitro differentiated MDDC from cryopreserved PBMC of cancer patients are functionally distinct from those of healthy donors we compared these cells for their expression of co-stimulatory and functional markers. In addition the effect of cryopreservation of PBMC precursors on the quality of MDDC was also evaluated using samples .

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