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báo cáo khoa học: " Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing | Xin et al. BMC Plant Biology 2011 11 61 http www.biomedcentral.com 1471-2229 11 61 BMC Plant Biology RESEARCH ARTICLE Open Access Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing A I I z t m I z t VI 1 2 t 1 I A s 1 2 t 71 X ĩ IL . 1 2 i I c z- . x 1 2 7 p. z m z i I_11 I 1 2 Ch -J s I 1 2 p. z I I V I z 1 2 Mingming Ain Yu Wang Yingyin Yao Na song Zhaorong Hu Danaan Qin Chaojie Ale Huiru Peng1 2 Zhongfu Ni1 2 and Qixin Sun1 2 3 Abstract Background Biotic and abiotic stresses such as powdery mildew infection and high temperature are important limiting factors for yield and grain quality in wheat production. Emerging evidences suggest that long non-protein coding RNAs npcRNAs are developmentally regulated and play roles in development and stress responses of plants. However identification of long npcRNAs is limited to a few plant species such as Arabidopsis rice and maize no systematic identification of long npcRNAs and their responses to abiotic and biotic stresses is reported in wheat. Results In this study by using computational analysis and experimental approach we identified 125 putative wheat stress responsive long npcRNAs which are not conserved among plant species. Among them some were precursors of small RNAs such as microRNAs and siRNAs two long npcRNAs were identified as signal recognition particle SRP 7S RNA variants and three were characterized as U3 snoRNAs. We found that wheat long npcRNAs showed tissue dependent expression patterns and were responsive to powdery mildew infection and heat stress. Conclusion Our results indicated that diverse sets of wheat long npcRNAs were responsive to powdery mildew infection and heat stress and could function in wheat responses to both biotic and abiotic stresses which provided a starting point to understand their functions and regulatory mechanisms in the future. Background The .