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Production and characterization of multifunctional endoxylanase by Bacillus sp. X13

The enzyme was highly inhibited by urea, and fairly inhibited by Triton X-100, CaCl2, ZnCl2, KCl, Na2SO3 PMSF, and 2-Mercaptoethanol. The properties of the enzyme presented in this study suggest that this enzyme could be a potential industrial interest. | Turk J Biol 33 (2009) 231-237 © TÜBİTAK doi:10.3906/biy-0806-18 Production and characterization of multifunctional endoxylanase by Bacillus sp. X13 Ashabil AYGAN1, Burhan ARIKAN2 1Kahramanmaraş Sütçü İmam University, Faculty of Science and Letters, Department of Biology, K.Maraş - TURKEY 2Çukurova University, Faculty of Science and Letters, Department of Biology, Molecular Biology Laboratory, Adana - TURKEY Received: 17.06.2008 Abstract: A multifunctional endoxylanase producing Bacillus sp. was isolated from soil. Enzyme synthesis occurred at temperatures between 20 °C and 60 °C with an optimum 37 °C. Analysis of the enzyme by SDS-PAGE revealed 4 active enzyme bands, which were estimated to be 66.5 kDa, 80.6 kDa, 95.5 kDa, and 108.4 kDa. The enzyme has a broad temperature range, between 20 and 90 °C, with an optimum at 40 °C; and maximum activity was at pH 6.0. The enzyme showed a gradual decrease in the remaining activity as the pre-incubation temperature increase. Thermostability was not 2+ also increased in the presence of Ca . An average of 71% of remaining activity observed when the enzyme incubated between pH 3.6 and 10 for 1 h. The enzyme was highly inhibited by urea, and fairly inhibited by Triton X-100, CaCl2, ZnCl2, KCl, Na2SO3 PMSF, and 2-Mercaptoethanol. The properties of the enzyme presented in this study suggest that this enzyme could be a potential industrial interest. Key words: Bacillus sp., extracellular, endoxylanase, xylanase, multifunctional Bacillus sp. X13 suşundan multifonksiyonel endoksilanaz üretimi ve karakterizasyonu Özet: Topraktan multifonksiyonel endoksilanaz üreten Bacillus sp. X13 suşu izole edilmiştir. Enzim sentezi optimum 37 °C olmak üzere 20 ile 60 °C arasında gerçekleşmiştir. SDS-PAGE analizinde, 66,5 kDa, 80,6 kDa, 95,5 kDa ve 108,4 kDa olarak hesaplanan 4 aktif enzim bandı gözlenmiştir. Enzim optimum aktivitesi 40 °C olup 20-90 °C arasında geniş bir sıcaklık aralığına sahiptir ve maksimum aktivitesi ise pH 6,0 olarak .