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Isolation and characterization of yeast from tomato plants for biological control of Alternaria solani

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Nine different yeast isolates from various parts of tomato plant were isolated from two different locations of Bengaluru. The population on tomato leaves varied from 2.01-3.5 x104 cfu/cm², on stems from 6.4-7.31 x104 cfu/g fresh weight and on fruits from 6.7-7.38 x104 cfu/g fresh weight. The yeast isolates were characterized for utilization of different carbon sources. Out of nine isolates, four yeast isolates TPL-I, RTPL, RTPS- II and RTPFII produced lipase enzyme. Further, they were evaluated for biocontrol activity against Alternaria solani in in vitro conditions. Among the yeast isolates, TPL-I yeast isolate showed strongly positive result by forming maximum zone of inhibition (9mm), produced minimum dry mycelial weight (40.07 mg) of pathogen and also showed highest percentage of inhibition of conidial germination (80.50 %) compared to other yeast isolates. Four yeast isolates produced relatively higher concentrations of IAA ranging from 0.21-0.68 μg/ml and the maximum concentration of IAA was produced by TPL- I. | Isolation and characterization of yeast from tomato plants for biological control of Alternaria solani

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