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Nghiên cứu điều chế vi hạt albumin gắn đồng vị phóng xạ Yttrium-90

Bài viết trình bày việc xác định các điều kiện tối ưu hoàn thành quy trình đánh dấu các vi hạt albumin với đồng vị phóng xạ Yttrium - 90 và hoàn thành quy trình kiểm tra chất lượng sản phẩm. | NGHIÊN CỨU ĐIỀU CHẾ VI HẠT ALBUMIN NGHIÊN CỨU KHOA HỌC GẮN ĐỒNG VỊ PHÓNG XẠ YTTRIUM-90 Study On Radiolabeling Of Albumin Particles With SCIENTIFIC RESEARCH Yttrium-90 Nguyễn Thị Khánh Giang Nguyễn Thị Ngọc Nguyễn Thanh Bình Nguyễn Thị Thu SUMMARY Objective This report is intended to determine the optimum conditions of the radiolabeling of microaggregated albumin particles with Yttrium-90 radioisotope and the completion of the quality control procedures. Subjects and Methods The albumin microsphere kit was prepared in sodium phosphate buffer. The original solution includes 2 mg albumin particle and 0.5 mg stannous chloride dihydrate. The albumin particles size was ranged from 5 mm to 30 mm. The mixture was washed three times with phosphate buffer saline pH 7.2 by centrifugation and suspended in 0.5 M sodium acetate buffer pH 6. Yttrium-90 in 1.0 M acetic acid was collected from 90Sr 90Y generator. The labeling of the particles with 90 Y 185 MBq was performed at pH 5.5 in acetate buffer with agitating for 60 min at room temperature. The labeled albumin suspensions were centrifuged at 3000 rpm for 15 min. Labeling yields was calculated using centrifugation filtration and compared with paper chromatography which is developed in the Tris Acetic EDTA. In this system the unbound of Y-90 migrates to an Rf of 0.9-1.0 and the radiolabeled albumin particles remains at the point of origin Rf 0 . The size of 90Y-albumin particles was compared with the albumin particles in the original solution to be sure that they didn t change during the labeling treatment Results The radiolabeling yields were more than 80 at pH 5 5 the labeled compound was dialysis in phosphate buffer. The radiochemical purity was 98 the reaction time marked 60 minutes at room temperature about 240C . The labeled compound was dialysis in phosphate buffer. The product has a radioactivity of more than 98 . The product has been tested and the quality requirements of radioactive drugs. Conclusion The .