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Báo cáo y học: "Transduction of Cu, Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into human chondrocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Transduction of Cu, Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into human chondrocytes. | Available online http arthritis-research.eom content 8 4 R96 Open Access Research article Transduction of Cu Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into human chondrocytes Hyun Ah Kim1 Dae Won Kim2 Jinseu Park2 and Soo Young Choi2 Department of Internal Medicine Hallym University Sacred Heart Hospital 896 Pyongchondong Dongan-gu Anyang Kyunggi-do 431-070 Korea 2Department of Biomedical Sciences and Research Institute for Bioscience and Biotechnology Hallym University Chunchon 200-702 Korea Corresponding author Hyun Ah Kim kimha@hallym.ac.kr Received 6 Mar 2006 Revisions requested 4 Apr 2006 Revisions received 4 May 2006 Accepted 12 May 2006 Published 22 Jun 2006 Arthritis Research Therapy 2006 8 R96 doi 10.1186 ar1972 This article is online at http arthritis-research.com content 8 4 R96 2006 Kim et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract This study was performed to investigate the transduction of a full-length superoxide dismutase SOD protein fused to transactivator of transcription Tat into human chondrocytes and to determine the regulatory function of transduced Tat-SOD in the inflammatory cytokine induced catabolic pathway. The pTat-SOD expression vector was constructed to express the basic domain of HIV-1 Tat as a fusion protein with Cu Zn-SOD. We also purified histidine-tagged SOD without an HIV-1 Tat and Tat-GFP as control proteins. Cartilage samples were obtained from patients with osteoarthritis OA and chondrocytes were cultured in both a monolayer and an explant. For the transduction of fusion proteins cells explants were treated with a variety of concentrations of fusion proteins. The transduced protein was detected by fluorescein labeling western blotting and .