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Báo cáo y học: "A novel approach to measure the contribution of matrix metalloproteinase in the overall net proteolytic activity present in synovial fluids of patients with arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: A novel approach to measure the contribution of matrix metalloproteinase in the overall net proteolytic activity present in synovial fluids of patients with arthritis. | Available online http arthritis-research.eom content 8 4 R1 25 Research article A novel approach to measure the contribution of matrix metalloproteinase in the overall net proteolytic activity present in synovial fluids of patients with arthritis Nathalie Simard1 Gilles Boire2 Artur J de Brum-Fernandes2 and Yves St-Pierre1 Open Access 1INRS-Institut Armand-Frappier University of Quebec Laval Quebec Canada 2Division of Rheumatology Department of Medicine Centre hospitalier universitaire de Sherbrooke Université de Sherbrooke Quebec Canada Corresponding author Yves St-Pierre yves.st-pierre@iaf.inrs.ca Received 13 Apr 2006 Revisions requested 24 May 2006 Revisions received 13 Jun 2006 Accepted 14 Jul 2006 Published 19 Jul 2006 Arthritis Research Therapy 2006 8 R125 doi 10.1186 ar2014 This article is online at http arthritis-research.com content 8 4 R1 25 2006 Simard et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Despite decades of research only a very limited number of matrix metalloproteinase MMP inhibitors have been successful in clinical trials of arthritis. One of the central problems associated with this failure may be our inability to monitor the local activity of proteases in the joints since the integrity of the extracellular matrix results from an equilibrium between noncovalent 1 1 stoichiometric binding of protease inhibitors to the catalytic site of the activated forms of the enzymes. In the present work we have measured by flow cytometry the net proteolytic activity in synovial fluids SF collected from 95 patients with osteoarthritis and various forms of inflammatory arthritis including rheumatoid arthritis spondyloarthropathies and chronic juvenile arthritis. We found that SF of patients with .