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Báo cáo y học: "Chronic OVA allergen challenged Siglec-F deficient mice have increased mucus, remodeling, and epithelial "

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Chronic OVA allergen challenged Siglec-F deficient mice have increased mucus, remodeling, and epithelial. | Cho et al. Respiratory Research 2010 11 154 http respiratory-research.eom content 11 1 154 RESPIRATORY RESEARCH RESEARCH Open Access Chronic OVA allergen challenged Siglec-F deficient mice have increased mucus remodeling and epithelial Siglec-F ligands which are up-regulated by IL-4 and IL-13 Jae Youn Cho Dae Jae Song Alexa Pham Peter Rosenthal Marina Miller Shanna Dayan Taylor A Doherty Ajit Varki David H Broide Abstract Background In this study we examined the role of Siglec-F a receptor highly expressed on eosinophils in contributing to mucus expression airway remodeling and Siglec-F ligand expression utilizing Siglec-F deficient mice exposed to chronic allergen challenge. Methods Wild type WT and Siglec-F deficient mice were sensitized and challenged chronically with OVA for one month. Levels of airway inflammation eosinophils Siglec-F ligand expresion and remodeling mucus fibrosis smooth muscle thickness extracellular matrix protein deposition were assessed in lung sections by image analysis and immunohistology. Airway hyperreactivity to methacholine was assessed in intubated and ventilated mice. Results Siglec-F deficient mice challenged with OVA for one month had significantly increased numbers of BAL and peribronchial eosinophils compared to WT mice which was associated with a significant increase in mucus expression as assessed by the number of periodic acid Schiff positive airway epithelial cells. In addition OVA challenged Siglec-F deficient mice had significantly increased levels of peribronchial fibrosis total lung collagen area of peribronchial trichrome staining as well as increased numbers of peribronchial TGF-P1 cells and increased levels of expression of the extracellular matrix protein fibronectin compared to OVA challenged WT mice. Lung sections immunostained with a Siglec-Fc to detect Siglec-F ligand expression demonstrated higher levels of expression of the Siglec-F ligand in the peribronchial region in OVA challenged Siglec-F deficient mice .