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Báo cáo y học: "Clinical and serological evaluation of a novel CENP-A peptide based ELISA"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Clinical and serological evaluation of a novel CENP-A peptide based ELISA. | Mahler et al. Arthritis Research Therapy 2010 12 R99 http arthritis-research.eom content 12 3 R99 RESEARCH ARTICLE Open Access Clinical and serological evaluation of a novel CENP-A peptide based ELISA Michael Mahler1 2 Liesbeth Maes3 Daniel Blockmans3 Rene Westhovens3 Xavier Bossuyt3 Gabriela Riemekasten4 Sandra Schneider4 Falk Hiepe4 Andreas Swart5 Irmgard Gurtler5 Karl Egerer6 Margrit Fooke1 2 and Marvin J Fritzler 7 Abstract Introduction Anti-centromere antibodies ACA are useful biomarkers in the diagnosis of systemic sclerosis SSc . ACA are found in 20 to 40 of SSc patients and albeit with lower prevalence in patients with other systemic autoimmune rheumatic diseases. Historically ACA were detected by indirect immunofluorescence IIF on HEp-2 cells and confirmed by immunoassays using recombinant CENP-B. The objective of this study was to evaluate a novel CENP-A peptide eLisa. Methods Sera collected from SSc patients n 334 and various other diseases n 619 and from healthy controls n 175 were tested for anti-CENP-A antibodies by the novel CENP-A enzyme linked immunosorbent assay ELISA . Furthermore ACA were determined in the disease cohorts by IIF ImmunoConcepts Sacramento CA USA CENP-B ELISA Dr. Fooke EliA CENP Phadia Freiburg Germany and line-immunoassay LIA Mikrogen Neuried Germany . Serological and clinical associations of anti-CENP-A with other autoantibodies were conducted in one participating centre. Inhibition experiments with either the CENP-A peptide or recombinant CENP-B were carried out to analyse the specificity of anti-CENP-A and -B antibodies. Results The CENP-A ELISA results were in good agreement with other ACA detection methods. According to the kappa method the qualitative agreements were 0.73 vs. IIF 0.81 vs. LIA 0.86 vs. CENP-B ELISA and 0.97 vs. EliA CENP . The quantitative comparison between CENP-A and CENP-B ELISA using 265 samples revealed a correlation value of rho 0.5 by Spearman equation . The receiver operating characteristic analysis