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Báo cáo y học: "Staphylococcus aureus enterotoxins induce IL-8 secretion by human nasal epithelial cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Staphylococcus aureus enterotoxins induce IL-8 secretion by human nasal epithelial cells. | Respiratory Research BioMed Central Research Staphylococcus aureus enterotoxins induce IL-8 secretion by human nasal epithelial cells Garrett J O Brien Gareth Riddell J Stuart Elborn Madeleine Ennis and Grzegorz Skibinski Open Access Address Respiratory Research Group School of Medicine and Dentistry Queen s University Belfast Grosvenor Road Belfast BT12 6BJ Northern Ireland UK Email Garrett J O Brien - obriengarrett@yahoo.ie Gareth Riddell - garethriddell@yahoo.com J Stuart Elborn - Stuart.Elborn@bch.n-i.nhs.uk Madeleine Ennis - m.ennis@qub.ac.uk Grzegorz Skibinski - g.skibinski@qub.ac.uk Corresponding author Published 04 September 2006 Received 21 December 2005 Accepted 04 September 2006 Respiratory Research 2006 7 115 doi l0.ll86 l 465-9921-7-115 This article is available from http respiratory-research.cOm content 7 1 1 15 2006 O Brien et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Staphylococcus aureus produces a set of proteins which act both as superantigens and toxins. Although their mode of action as superantigens is well understood little is known about their effects on airway epithelial cells. Methods To investigate this problem primary nasal epithelial cells derived from normal and asthmatic subjects were stimulated with staphylococcal enterotoxin A and B SEA and SEB and secreted supernatants and cell-associated cell lysates IL-8 TNF-a RANTES and eotaxin were determined by specific ELISAs. Results Non-toxic concentrations of SEA and SEB 0.01 pg ml and 1.0 pg ml induced IL-8 secretion after 24 h of culture. Pre-treatment of the cells with IFN-y 50 IU ml resulted in a further increase of IL-8 secretion. In cells from healthy donors pretreated with IFN-y SEA at 1.0 pg ml induced release of