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Báo cáo y học: " Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione. | Respiratory Research BioMed Central Research Open Access Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro role of glutathione Fabrizio Luppi1 Jamil Aarbiou1 Sandra van Wetering1 Irfan Rahman2 Willem I de Boer1 Klaus F Rabe1 and Pieter S Hiemstra 1 Address Department of Pulmonology Leiden University Medical Center P.O. Box 9600 2300RC Leiden The Netherlands and 2Department of Environmental Medicine Division of Lung Biology and Disease University of Rochester Medical Center Rochester NY 14642 USA Email Fabrizio Luppi - luppi.fabrizio@unimo.it Jamil Aarbiou - j.aarbiou@erasmusmc.nl Sandra van Wetering - s.wetering@pharming.com Irfan Rahman - Irfan_rahman@urmc.rochester.edu Willem I de Boer - deboer.pim@hetnet.nl Klaus F Rabe - k.f.rabe@lumc.nl Pieter S Hiemstra - p.s.hiemstra@lumc.nl Corresponding author Published 25 November 2005 Received 13 May 2005 Accepted 25 November 2005 Respiratory Research 2005 6 140 doi l0.ll 86 1465-9921-6-140 This article is available from http respiratory-research.com content 6 1 140 2005 Luppi et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Increased airway epithelial proliferation is frequently observed in smokers. To elucidate the molecular mechanisms leading to these epithelial changes we studied the effect of cigarette smoke condensate CSC on cell proliferation wound closure and mitogen activated protein kinase MAPK activation. We also studied whether modulation of intracellular glutathione thiol levels could attenuate CSC-induced cell proliferation. Methods Cells of the bronchial epithelial cell line NCI-H292 and subcultures of primary bronchial epithelial cells were used for the present study. The effect