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Báo cáo y học: "Effects of PM10 in human peripheral blood monocytes and J774 macrophages"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Effects of PM10 in human peripheral blood monocytes and J774 macrophages. | Respiratory Research BioMed Central Research Open Access Effects of PM10 in human peripheral blood monocytes and J774 macrophages DM Brown 1 K Donaldson2 and V Stone1 Address 1School of Life Sciences Napier University Edinburgh UK and 2ELEGI Laboratory Wilkie Building University of Edinburgh UK Email DM Brown - da.brown@napier.ac.uk K Donaldson - da.brown@napier.ac.uk V Stone - v.stone@napier.ac.uk Corresponding author Published 21 December 2004 Received 29 September 2004 Accepted 21 December 2004 Respiratory Research 2004 5 29 doi 10.1186 1465-9921 -5-29 This article is available from http respiratory-research.cOm content 5 1 29 2004 Brown et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The effects of PM10 one of the components of particulate air pollution was investigated using human monocytes and a mouse macrophage cell line J774 . The study aimed to investigate the role of these nanoparticles on the release of the pro-inflammatory cytokine TNF-a and IL-1a gene expression. We also investigated the role of intracellular calcium signalling events and oxidative stress in control of these cytokines and the effect of the particles on the functioning of the cell cytoskeleton. We showed that there was an increase in intracellular calcium concentration in J774 cells on treatment with PM10 particles which could be significantly reduced with concomitant treatment with the calcium antagonists verapamil the intracellular calcium chelator BAPTA-AM but not with the antioxidant nacystelyn or the calmodulin inhibitor W-7. In human monocytes PM10 stimulated an increase in intracellular calcium which was reduced by verapamil BAPTA-AM and nacystelyn. TNF-a release was increased with particle treatment in human monocytes and