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Báo cáo y học: "Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells. | Respiratory Research BioMed Central Research Open Access Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells Xiu M Wei1 Henry S Kim1 Rakesh K Kumar1 Gavin J Heywood1 John E Hunt1 H Patrick McNeil1 and Paul S Thomas 1 2 Address 1Inflammation Research Unit School of Pathology Faculty of Medicine UNSW Sydney Australia and 2Department of Respiratory Medicine Prince of Wales Hospital Randwick NSW 2031 Australia Email Xiu M Wei - wxm1974@hotmail.com Henry S Kim - pinny_3@hotmail.com Rakesh K Kumar - r.kumar@unsw.edu.au Gavin J Heywood - gavinsteph@bigpond.com John E Hunt - j.hunt@unsw.edu.au H Patrick McNeil - p.mcneil@unsw.edu.au Paul S Thomas - paul.thomas@unsw.edu.au Corresponding author Published 19 September 2005 Received 28 June 2005 Respiratory Research 2005 6 108 doi 10.1186 1465-9921-6-108 Accepted 19 September 2005 This article is available from http respiratory-research.cOm content 6 1 108 2005 Wei et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Exhaled nitric oxide eNO is decreased by cigarette smoking. The hypothesis that oxides of nitrogen NOX in cigarette smoke solution CSS may exert a negative feedback mechanism upon NO release from epithelial AEC A549 and NHTBE and basophilic cells RBL-2H3 was tested in vitro. CSS inhibited both NO production and degranulation measured as release of betahexosaminidase in a dose-dependent manner from RBL-2H3 cells. Inhibition of NO production by CSS in AEC A549 and NHTBE cells was also dose-dependent. In addition CSS decreased expression of NOS mRNA and protein expression. The addition of NO inhibitors and scavengers did not however reverse the effects of CSS nor did a NO donor SNP or nicotine mimic CSS. N-acetyl-cysteine